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Aldolase A antibody

Anti-Aldolase A antibody used in Immunoprecipitation (IP). GTX26190
Anti-Aldolase A antibody used in Western Blot (WB). GTX26190
Anti-Aldolase A antibody used in Western Blot (WB). GTX26190
Anti-Aldolase A antibody used in Immunoprecipitation (IP). GTX26190
Anti-Aldolase A antibody used in Western Blot (WB). GTX26190

Cat. No. GTX26190

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Human, Rabbit
Package
100 μl ($399)
See all Aldolase A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
IP 1:100
ELISA 1:5000-1:20000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Aldolase from rabbit muscle

Purification

IgG fraction
This product was prepared from monospecific antiserum by a delipidation, salt fractionation and ion exchange chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldolase, fructose-bisphosphate A

Cellular Localization

Cytoplasm,Myofibril,Sarcomere,I band,M line

Database

Research Area

DATA IMAGES

Anti-Aldolase A antibody used in Immunoprecipitation (IP). GTX26190

GTX26190 IP Image

IP analysis of various samples using GTX26190 Aldolase A antibody.
Lane 1 : Purified aldolase
Lane 2 : Serum without aldolase
Lane 3 : Serum with 20 μL aldolase
Lane 4 : Serum with 80 μL aldolasee
Lane 5 : Serum with 120 μL aldolasee
Lane 6 : Serum with 150 μL aldolasee
Lane 7 : Serum with 300 μL aldolase

Anti-Aldolase A antibody used in Western Blot (WB). GTX26190

GTX26190 WB Image

WB analysis of A293 whole cell lysate using GTX26190 Aldolase A antibody.
Dilution : 1:1000

Anti-Aldolase A antibody used in Western Blot (WB). GTX26190

GTX26190 WB Image

WB analysis of various samples using GTX26190 Aldolase A antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Loading : 25 μg
Dilution : 1:1000

Anti-Aldolase A antibody used in Immunoprecipitation (IP). GTX26190

GTX26190 IP Image

IP analysis of various samples using GTX26190 Aldolase A antibody.
Lane 1 : Purified aldolase
Lane 2 : Serum without aldolase
Lane 3 : Serum with 100 μL aldolase
Lane 4 : Serum with 200 μL aldolase

Anti-Aldolase A antibody used in Western Blot (WB). GTX26190

GTX26190 WB Image

IgG purified antibody to rabbit muscle aldolase (GTX26190) was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4º C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399