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SEMA4B antibody

KO/KD
Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035
Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035
Anti-SEMA4B antibody used in Western Blot (WB). GTX121035
Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035
Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035
Anti-SEMA4B antibody used in Western Blot (WB). GTX121035
Anti-SEMA4B antibody used in Western Blot (WB). GTX121035
Anti-SEMA4B antibody used in Western Blot (WB). GTX121035

Cat. No. GTX121035

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SEMA4B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

U87-MG , Mouse brain

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human SEMA4B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731613

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

semaphorin 4B , SEMAC , SemC

Cellular Localization

Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035

GTX121035 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using SEMA4B(GTX121035) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035

GTX121035 IHC-P Image

SEMA4B antibody detects SEMA4B protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
SEMA4B antibody (GTX121035) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEMA4B antibody used in Western Blot (WB). GTX121035

GTX121035 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX121035 diluted at 1:1000

Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035

GTX121035 IHC-P Image

SEMA4B antibody detects SEMA4B protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SEMA4B antibody (GTX121035) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035

GTX121035 IHC-P Image

SEMA4B antibody detects SEMA4B protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SEMA4B antibody (GTX121035) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEMA4B antibody used in Western Blot (WB). GTX121035

GTX121035 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA4B antibody (GTX121035) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SEMA4B antibody used in Western Blot (WB). GTX121035

GTX121035 WB Image

KO/KD

The data was published in the journal Sci Rep in 2018.PMID: 29311693

Anti-SEMA4B antibody used in Western Blot (WB). GTX121035

GTX121035 WB Image

The data was published in the journal Sci Rep in 2018.PMID: 29311693

REFERENCE

Application Reference

Peretz L et al. Sci Rep 2018; 8 (1) : 93 Combined shRNA over CRISPR/cas9 as a methodology to detect off-target effects and a potential compensatory mechanism.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169