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KRT71 antibody [N3C3]

Anti-KRT71 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107343
Anti-KRT71 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107343
Anti-KRT71 antibody [N3C3] used in Western Blot (WB). GTX107343

Cat. No. GTX107343

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Sheep
Package
100 μl ($399),
25 μl ($169)
See all KRT71 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KRT71. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173791

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 71 , HYPT13 , K6IRS1 , KRT6IRS , KRT6IRS1

Background

K6IRS1 belongs to a family of type II keratins that are specifically expressed in the inner root sheath of hair follicles.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-KRT71 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107343

GTX107343 IHC-P Image

KRT71 antibody [N3C3] detects KRT71 protein at membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
KRT71 antibody [N3C3] (GTX107343) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KRT71 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107343

GTX107343 ICC/IF Image

KRT71 antibody [N3C3] detects KRT71 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KRT71 protein stained by KRT71 antibody [N3C3] (GTX107343) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-KRT71 antibody [N3C3] used in Western Blot (WB). GTX107343

GTX107343 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX107343 diluted at 1:5000

REFERENCE

Application Reference

Shanhe Wang et al. Front Cell Dev Biol 2021; 9 : 800157 Single-Cell Transcriptomics Reveals the Molecular Anatomy of Sheep Hair Follicle Heterogeneity and Wool Curvature.
Reactivity : Sheep
Hu T et al. Genes (Basel) 2021; 12 (12) miR-143 Targeting CUX1 to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep.
Application : WB
Reactivity : Human
Li G et al. Front Mol Neurosci 2019; 12 : 212 Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations.
Application : ICC/IF
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169