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ASB8 antibody

Anti-ASB8 antibody used in Western Blot (WB). GTX117973
Anti-ASB8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117973
Anti-ASB8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117973
Anti-ASB8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117973
Anti-ASB8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117973

Cat. No. GTX117973

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all ASB8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Mouse, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASB8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730873

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ankyrin repeat and SOCS box containing 8 , PP14212

Cellular Localization

Cytoplasm

Background

May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Database

Research Area

DATA IMAGES

Anti-ASB8 antibody used in Western Blot (WB). GTX117973

GTX117973 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX117973 diluted at 1:1000

Anti-ASB8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117973

GTX117973 IHC-P Image

ASB8 antibody detects ASB8 protein at cytoplasm in rat skin by immunohistochemical analysis.
Sample: Paraffin-embedded rat skin.
ASB8 antibody (GTX117973) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ASB8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117973

GTX117973 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ASB8(GTX117973) antibody at 1:500 dilution.

Anti-ASB8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117973

GTX117973 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ASB8(GTX117973) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ASB8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117973

GTX117973 IHC-P Image

ASB8 antibody detects ASB8 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ASB8 antibody (GTX117973) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169