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Hsp90 alpha antibody

Anti-Hsp90 alpha antibody used in Immunoprecipitation (IP). GTX22928
Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928
Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928
Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX22928
Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928
Anti-Hsp90 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22928
Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX22928
Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX22928
Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX22928
Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928
Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928

Cat. No. GTX22928

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Primate
Package
100 μg ($399)
See all Hsp90 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50-1:200
IHC-P 5 μg/ml
IP 2 μg
IHC Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Product Note

This antibody does not detect HSP84.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90, alpha (cytosolic), class A member 1 , 86kDa , 89kDa , AL024080 , AL024147 , Hsp86-1 , Hsp89 , Hsp90 , Hspca , hsp4

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, Ah receptor, and some kinases. Studies have shown that murine HSP90 exists as two forms, HSP84 and HSP86, coded by related but separate genes, with 86% homologous amino acid sequences. These forms are analogous to the two forms of human HSP90, HSP89 beta and HSP89 alpha. In an unstressed mouse fibroblast, the basal level of HSP84 is found to be double that of HSP86. However, after heat shock, HSP86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP86, but not HSP84, decreases. HSP84 and HSP86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form.

Database

Research Area

DATA IMAGES

Anti-Hsp90 alpha antibody used in Immunoprecipitation (IP). GTX22928

GTX22928 IP Image

IP analysis of HeLa cell lysates using GTX22928 Hsp90 alpha antibody.
GTX22928 was used as IP and IB antibody
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928

GTX22928 WB Image

WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22928 Hsp90 alpha antibody.
Dilution : 1:1000

Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928

GTX22928 WB Image

WB analysis of Hsp86 (Hsp90 alpha) and Hsp84 (Hsp90 beta) using GTX22928 Hsp90 alpha antibody. (left) or GTX79247 HSP90 beta antibody respectively

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX22928

GTX22928 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22928 Hsp90 alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928

GTX22928 WB Image

WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22928 Hsp90 alpha antibody.
Dilution : 1:1000

Anti-Hsp90 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22928

GTX22928 ICC/IF Image

ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX22928 Hsp90 alpha antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX22928

GTX22928 IHC-P Image

IHC-P analysis of differentiated colon adenocarcinoma tissue from human colon tissue sections using GTX22928 Hsp90 alpha antibody.
Left : without primary antibody
Right : with primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC
Dilution : 1:100

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX22928

GTX22928 IHC-P Image

IHC-P analysis of human kidney tissue using GTX22928 Hsp90 alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX22928

GTX22928 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX22928 Hsp90 alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928

GTX22928 WB Image

WB analysis of 50 ug of the indicated whole cell lysates using GTX22928 Hsp90 alpha antibody.
Hsp86 also known as Hsp90 alpha
Dilution : 1:1000

Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX22928

GTX22928 WB Image

WB analysis of A431 cell lysate (Lane 3~6) using GTX22928 Hsp90 alpha antibody. Lanes 1 and 2 are control samples of purified, recombinant Hsp86 (Hsp90 alpha) and Hsp84 (Hsp90 beta), respectively.
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399