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Mouse LDHA protein, His tag (active)

Mouse LDHA protein, His tag (active). GTX67082-pro

Cat. No. GTX67082-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($219)
See all LDHA protein products

APPLICATION

Application Note

Specific activity is > 250 units/mg, and is defined as the amount of enzyme that convert 1.0 μmole of pyruvate to L-lactate and per minute at pH 7.5 at 37ºC.

Observed MW

28-40 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, C-terminal His-Tag; Length: 340 a.a. Sequence: MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHHHHH

Expression System

Baculovirus

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lactate dehydrogenase A , Ldh1 , Ldhm , l7R2

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Mouse LDHA protein, His tag (active). GTX67082-pro

GTX67082-pro Image

3 μg of GTX67082-pro Mouse LDHA protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 219