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NSMCE1 antibody [N1C3]

Anti-NSMCE1 antibody [N1C3] used in Western Blot (WB). GTX107136
Anti-NSMCE1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107136

Cat. No. GTX107136

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NSMCE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSMCE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951030

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NSE1 homolog, SMC5-SMC6 complex component , NSE1

Cellular Localization

Nucleus

Background

Probable component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.

Database

Research Area

DATA IMAGES

Anti-NSMCE1 antibody [N1C3] used in Western Blot (WB). GTX107136

GTX107136 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX107136 diluted at 1:2000

Anti-NSMCE1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107136

GTX107136 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using NSMCE1(GTX107136) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Dupont L et al. Cell Host Microbe 2021; 29 (5) : 792-805.e6 The SMC5/6 complex compacts and silences unintegrated HIV-1 DNA and is antagonized by Vpr.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169