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MCP1 / CCL2 antibody [ECE.2]

Cat. No. GTX54381

Host

 Rat

Clonality

 Monoclonal

Clone Name

 ECE.2

Isotype

 IgG1

Application

 WB, IHC-Fr

Reactivity

 Mouse
Package
100 μg ($399)
See all MCP1 / CCL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.8 μg/ml
IHC-Fr Assay dependent

Note :

WB
Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk.

IHC-Fr
Sections (6 μm) were fixed with 4% PFA, blocked using 0.1% Triton-X and 5% serum for 15min at 37ºC or o/n 4 ºC and then incubated with 2 μg/ml antibody for 2h at 37ºC.
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Product Note

We do not recommend use of this product for Human samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues 102-130 of mouse MCP-1

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chemokine (C-C motif) ligand 2 , AI323594 , HC11 , JE , MCAF , MCP-1 , MCP1 , SMC-CF , Scya2 , Sigje

Cellular Localization

Secreted

Background

This gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. [provided by RefSeq, Sep 2015]

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 399