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Opsin 5 antibody [N2C1], Internal

Anti-Opsin 5 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100173
Anti-Opsin 5 antibody [N2C1], Internal used in Western Blot (WB). GTX100173

Cat. No. GTX100173

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Opsin 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Opsin 5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1081004

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

opsin 5 , GPR136 , GRP136 , PGR12 , TMEM13

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. This gene belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this gene may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Opsin 5 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100173

GTX100173 IHC-Fr Image

Opsin 5 antibody [N2C1], Internal detects Opsin 5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Opsin 5 protein stained by Opsin 5 antibody [N2C1], Internal (GTX100173) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Opsin 5 antibody [N2C1], Internal used in Western Blot (WB). GTX100173

GTX100173 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3 (GTX14654)
10% SDS PAGE
GTX100173 diluted at 1:1000

REFERENCE

Application Reference

Y Lan et al. Br J Dermatol 2021; 185 (2) : 391-404 Opsin 5 is a key regulator of ultraviolet radiation-induced melanogenesis in human epidermal melanocytes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169