TMED4 antibody [N1C3]

Name: TMED4 antibody [N1C3]
Brand: GeneTex
SKU: GTX112712
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-TMED4 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX112712

Anti-TMED4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX112712

Anti-TMED4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112712

Anti-TMED4 antibody [N1C3] used in Western Blot (WB). GTX112712

Cat. No. GTX112712

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Wm
Reactivity	Human, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all TMED4 products

Datasheet
Document

View product citations for antibody GTX112712 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMED4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620429

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane p24 trafficking protein 4 , ERS25 , GMP25iso , HNLF , p24a3 , p24alpha3

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis.

Database

Gene ID: 222068 TMED4
UniProt: Q7Z7H5 TMED4

Research Area

DATA IMAGES

GTX112712 IHC-Wm Image

TMED4 antibody [N1C3] detects Tmed4 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
TMED4 antibody [N1C3] (GTX112712) dilution: 1:50.

GTX112712 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using TMED4(GTX112712) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112712 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using TMED4(GTX112712) antibody at 1:200 dilution.

GTX112712 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMED4 antibody [N1C3] (GTX112712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW