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ZIC2 antibody

Anti-ZIC2 antibody used in Western Blot (WB). GTX10550
Anti-ZIC2 antibody used in Western Blot (WB). GTX10550

Cat. No. GTX10550

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Mouse, Rat
Package
50 μg ($399)
See all ZIC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:10000-1:50000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to an internal region near amino acids 180-210 of Mouse ZIC-2.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein of the cerebellum 2 , HPE5 , Ku

Background

ZIC2 (zinc finger protein of the cerebellum 2) is a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. In mouse, knockout of Zic1 leads to aplasia of cerebellum and skeletal abnormalities. Mutation in Zic2 and Zic3 in mouse and/or human cause holoprosencephaly and heterotaxis, respectively. Holoprosencephaly is the most common structural anomaly of the human brain.

Database

Research Area

DATA IMAGES

Anti-ZIC2 antibody used in Western Blot (WB). GTX10550

GTX10550 WB Image

Western blot using GeneTex's Affinity Purified anti-ZIC2 antibody shows detection of a band ~55 kDa (arrowhead) corresponding to ZIC2 in lysates from (lane 1) mouse brain and (lane 2) rat brain. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-ZIC2 antibody. Signal was detected using standard techniques. The upper band in the mouse brain lysate represents Zic2.

Anti-ZIC2 antibody used in Western Blot (WB). GTX10550

GTX10550 WB Image

WB analysis of various samples using GTX10550 ZIC2 antibody.
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Loading : 20 μg
Dilution : 1:500

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399