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SynGAP antibody

Anti-SynGAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23344
Anti-SynGAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23344
Anti-SynGAP antibody used in IHC (Paraffin sections) (IHC-P). GTX23344
Anti-SynGAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23344

Cat. No. GTX23344

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all SYNGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 2 μg/ml
IHC-P 1:100
FACS 3-5 μg/10⁶ cells
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

GST fusion protein of a recombinant fragment, residues 947-1167, of rat SynGAP.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptic Ras GTPase activating protein 1 homolog (rat) , Gm1963 , Syngap

Cellular Localization

Membrane,Cell junction, synapse

Background

This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Homozygous mutations result in early post-embryonic lethality, while heterozygous mutant mice display a variety of phenotypes that include learning and memory defects, hyperactivity, and audiogenic seizures. [provided by RefSeq, Nov 2016]

Database

Research Area

DATA IMAGES

Anti-SynGAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23344

GTX23344 ICC/IF Image

ICC/IF analysis of rat hippocampal neurons using GTX23344 SynGAP antibody.

Anti-SynGAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23344

GTX23344 ICC/IF Image

ICC/IF analysis of rat hippocampal neurons using GTX23344 SynGAP antibody.

Anti-SynGAP antibody used in IHC (Paraffin sections) (IHC-P). GTX23344

GTX23344 IHC-P Image

IHC-P analysis of human heart tissue using GTX23344 SynGAP antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-SynGAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23344

GTX23344 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX23344 SynGAP antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399