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Rat EGF protein, His and GST tag (active)

Rat EGF protein, His and GST tag (active). GTX00373-pro
Rat EGF protein, His and GST tag (active). GTX00373-pro
Rat EGF protein, His and GST tag (active). GTX00373-pro

Cat. No. GTX00373-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($259)
See all EGF protein products

APPLICATION

Application Note

Epidermal growth factor (EGF) binding to EGFR, results in cellular proliferation, differentiation, and survival. To test the effect of EGF on cell proliferation, 3T3 fibroblasts cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM  prior to the addition of various concentrations of recombinant rat EGF. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of 3T3 cells after incubation with EGF for 96h observed by inverted microscope.

Observed MW

38 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Asn974~Arg1026 (NP_036974.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor

Background

binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat EGF protein, His and GST tag (active). GTX00373-pro

GTX00373-pro Functional Assay Image

Cell proliferation effect of GTX00373-pro Rat EGF protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated 3T3 cells cultured in DMEM for 96hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 1 ng/ml EGF for 96hrs.

Rat EGF protein, His and GST tag (active). GTX00373-pro

GTX00373-pro Image

WB analysis of GTX00373-pro Rat EGF protein (active).

Rat EGF protein, His and GST tag (active). GTX00373-pro

GTX00373-pro Image

SDS-PAGE analysis of GTX00373-pro Rat EGF protein (active).

REFERENCE

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SDS
Proclin.pdf
Package List Price ($)
$ 259