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EBF3 antibody [C1C3]

Anti-EBF3 antibody [C1C3] used in Immunoprecipitation (IP). GTX117656
Anti-EBF3 antibody [C1C3] used in Western Blot (WB). GTX117656
Anti-EBF3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117656

Cat. No. GTX117656

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EBF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EBF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619628

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EBF transcription factor 3 , COE3 , EBF-3 , HADDS , O/E-2 , OE-2

Cellular Localization

Nucleus

Background

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.

Database

Research Area

DATA IMAGES

Anti-EBF3 antibody [C1C3] used in Immunoprecipitation (IP). GTX117656

GTX117656 IP Image

Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 μg of EBF3 antibody [C1C3] (GTX117656).
Western blot analysis was performed using EBF3 antibody [C1C3] (GTX117656) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-EBF3 antibody [C1C3] used in Western Blot (WB). GTX117656

GTX117656 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX117656 diluted at 1:1000

Anti-EBF3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117656

GTX117656 ICC/IF Image

EBF3 antibody [C1C3] detects EBF3 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EBF3 protein stained by EBF3 antibody [C1C3] (GTX117656) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169