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APC13 antibody

Anti-APC13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85186
Anti-APC13 antibody used in Western Blot (WB). GTX85186
Anti-APC13 antibody used in Western Blot (WB). GTX85186

Cat. No. GTX85186

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

9 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

APC13 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC13.The immunogen is located within the last 50 amino acids of APC13.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

anaphase promoting complex subunit 13 , APC13 , SWM1

Cellular Localization

Nucleus

Background

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C.

Database

Research Area

DATA IMAGES

Anti-APC13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85186

GTX85186 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX85186 APC13 antibody.
Working concentration : 20 μg/ml

Anti-APC13 antibody used in Western Blot (WB). GTX85186

GTX85186 WB Image

WB analysis of Jurkat cell tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85186 APC13 antibody.
Working concentration : 1 μg/ml

Anti-APC13 antibody used in Western Blot (WB). GTX85186

GTX85186 WB Image

WB analysis of 25ng APC13 recombinant protein using GTX85186 APC13 antibody.
Working concentration : 1μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399