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DECR2 antibody

Anti-DECR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115788
Anti-DECR2 antibody used in Western Blot (WB). GTX115788
Anti-DECR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115788

Cat. No. GTX115788

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DECR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DECR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620714

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2,4-dienoyl-CoA reductase 2 , PDCR , SDR17C1

Cellular Localization

Peroxisome

Background

Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid.

Database

Research Area

DATA IMAGES

Anti-DECR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115788

GTX115788 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using DECR2(GTX115788) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DECR2 antibody used in Western Blot (WB). GTX115788

GTX115788 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX115788 diluted at 1:1000

Anti-DECR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115788

GTX115788 ICC/IF Image

DECR2 antibody detects DECR2 protein at peroxisome by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DECR2 protein stained by DECR2 antibody (GTX115788) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169