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C1orf77 antibody, Internal

Anti-C1orf77 antibody, Internal used in Immunoprecipitation (IP). GTX45303
Anti-C1orf77 antibody, Internal used in Western Blot (WB). GTX45303
Anti-C1orf77 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX45303

Cat. No. GTX45303

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
50 μg ($409)
See all C1orf77 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
IP Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

THP-1

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human C1orf77

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromatin target of PRMT1 , C10orf77 , C1orf77 , FL-SRAG , FOP , SRAG , SRAG-3 , SRAG-5 , pp7704

Cellular Localization

Nucleus,nucleoplasm,Nucleus speckle,Nucleolus

Background

This gene encodes a small nuclear protein that is characterized by an arginine and glycine rich region. This protein may have an important role in the regulation of fetal globin gene expression and in the activation of estrogen-responsive genes. A recent study reported that this protein binds 5-hydroxymethylcytosine (5hmC) and associates with an arginine methyltransferase complex (methylosome), which promotes methylation of arginine 3 of histone H4 (H4R3) and activation of genes involved in glioblastomagenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-C1orf77 antibody, Internal used in Immunoprecipitation (IP). GTX45303

GTX45303 IP Image

IP analysis of mouse brain tissue using GTX45303 C1orf77 antibody at 1:500. IP antibody: 6μg.

Anti-C1orf77 antibody, Internal used in Western Blot (WB). GTX45303

GTX45303 WB Image

WB analysis of THP-1 cells using GTX45303 C1orf77 antibody at 0.2-1μg/ml.

Anti-C1orf77 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX45303

GTX45303 IHC-P Image

IHC-P analysis of human brain stems (NT2) cells using GTX45303 C1orf77 antibody at 1:500.
Red: C1orf77
Blue: DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409