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Glycine dehydrogenase antibody [N3C2-2], Internal

Comparison
Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in Western Blot (WB). GTX110267
Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in Immunoprecipitation (IP). GTX110267
Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110267
Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110267
Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110267
Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110267

Cat. No. GTX110267

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Glycine dehydrogenase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glycine dehydrogenase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950407

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycine decarboxylase , GCE , GCSP , HYGN1

Cellular Localization

Mitochondrion

Background

The enzyme system for cleavage of glycine (glycine cleavage system; GCS; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes; see MIM 238310, MIM 238330, and MIM 238331.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in Western Blot (WB). GTX110267

GTX110267 WB Image

Comparison

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:1000 and competitor's antibody (CST#12794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in Immunoprecipitation (IP). GTX110267

GTX110267 IP Image

Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 μg of Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).
Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110267

GTX110267 IHC-P Image

Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110267

GTX110267 IHC-P Image

Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110267

GTX110267 IHC-P Image

Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glycine dehydrogenase antibody [N3C2-2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110267

GTX110267 ICC/IF Image

Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min.
Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:400.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169