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Lactoperoxidase antibody (HRP)

Anti-Lactoperoxidase antibody (HRP) used in Western Blot (WB). GTX40571
Anti-Lactoperoxidase antibody (HRP) used in Western Blot (WB). GTX40571

Cat. No. GTX40571

Host

 Sheep

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, Dot, ELISA

Reactivity

 Bovine
Package
1 mg ($399)
See all Lactoperoxidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
IP 1:100
Dot Assay dependent
ELISA 1:5000-1:20000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Lactoperoxidase collected from bovine milk

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lactoperoxidase

Cellular Localization

Secreted

Background

Lactoperoxidase is a natural antibacterial agent in bovine milk, a medium otherwise rich in its ability to support bacterial growth. Milk lactoperoxidase levels vary among species, ranging from little or none in humans to very high concentrations in guinea pigs. Interestingly, lactoperoxidase from milk is identical to the lacrimal and salivary forms of the enzyme. In addition, the enzyme catalyzes the hydrogen peroxide oxidation of iodine and can be employed in labelling proteins with radioiodine.

Database

Research Area

DATA IMAGES

Anti-Lactoperoxidase antibody (HRP) used in Western Blot (WB). GTX40571

GTX40571 WB Image

Genetex Sheep anti Lactoperoxidase antibody was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C ).

Anti-Lactoperoxidase antibody (HRP) used in Western Blot (WB). GTX40571

GTX40571 WB Image

WB analysis of reduced (R) and non-reduced (NR) bovine lactoperoxidase protein using GTX40571 Lactoperoxidase antibody (HRP).
Loading : 1 μg
Dilution : 1:3000

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Package List Price ($)
$ 399