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RGMB antibody [N3C3]

Anti-RGMB antibody [N3C3] used in Western Blot (WB). GTX107091
Anti-RGMB antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107091
Anti-RGMB antibody [N3C3] used in Western Blot (WB). GTX107091

Cat. No. GTX107091

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RGMB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Mouse brain , A431 , H1299 , HeLaS3

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGMB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951699

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

repulsive guidance molecule BMP co-receptor b , DRAGON

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor

Background

RGMB is a glycosylphosphatidylinositol (GPI)-anchored member of the repulsive guidance molecule family (see RGMA, MIM 607362) and contributes to the patterning of the developing nervous system (Samad et al., 2005 [PubMed 15671031]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RGMB antibody [N3C3] used in Western Blot (WB). GTX107091

GTX107091 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:H1299
C:HeLa S3(GTX14654)
10% SDS PAGE
GTX107091 diluted at 1:1000

Anti-RGMB antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107091

GTX107091 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RGMB(GTX107091) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RGMB antibody [N3C3] used in Western Blot (WB). GTX107091

GTX107091 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX107091 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169