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Rat Coagulation Factor II protein, His tag

Rat Coagulation Factor II protein, His tag. GTX00058-pro
Rat Coagulation Factor II protein, His tag. GTX00058-pro
Rat Coagulation Factor II protein, His tag. GTX00058-pro

Cat. No. GTX00058-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($359)
See all Thrombin products

APPLICATION

Application Note

Coagulation Factor II (F2) also commonly called pro-thrombin is a coagulation protein in the blood stream that has many effects in the coagulation cascade. It is a serine protease that converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Besides, Protein C Inhibitor (PCI) has been identified as an interactor of F2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat F2 and recombinant rat PCI. Briefly, F2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PCI-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-F2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of F2 and PCI was in a dose dependent manner.

Observed MW

20 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser201~Arg323

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

coagulation factor II

Background

catalyzes the preferential cleavage of Arg-Gly; activates fibrinogen to fibrin and releases fibrinopeptides A and B; involved in blood coagulation and wound repair [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Coagulation Factor II protein, His tag. GTX00058-pro

GTX00058-pro Functional Assay Image

Functional ELISA analysis of GTX00058-pro Rat Coagulation Factor II protein which can bind immobilized PCI protein.

Rat Coagulation Factor II protein, His tag. GTX00058-pro

GTX00058-pro Image

WB analysis of GTX00058-pro Rat Coagulation Factor II protein.

Rat Coagulation Factor II protein, His tag. GTX00058-pro

GTX00058-pro Image

SDS-PAGE analysis of GTX00058-pro Rat Coagulation Factor II protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 359