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PU.1/Spi1 antibody

Anti-PU.1/Spi1 antibody used in IHC (Whole mount) (IHC-Wm). GTX128266
Anti-PU.1/Spi1 antibody used in IHC (Whole mount) (IHC-Wm). GTX128266

Cat. No. GTX128266

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish PU.1/Spi1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish PU.1/Spi1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885743

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Spi-1 proto-oncogene b , cb1086 , pu.1 , pu1 , sfpi1 , si:by184l24.2 , spi1

Database

Research Area

DATA IMAGES

Anti-PU.1/Spi1 antibody used in IHC (Whole mount) (IHC-Wm). GTX128266

GTX128266 IHC-Wm Image

PU.1/Spi1 antibody detects PU.1/Spi1 protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
PU.1/Spi1 antibody (GTX128266) dilution: 1:200.
Anti-PU.1/Spi1 antibody used in IHC (Whole mount) (IHC-Wm). GTX128266

GTX128266 IHC-Wm Image

The data was published in the 2022 in Cells. PMID: 35159152

REFERENCE

Application Reference

Reuter H et al. Cells 2022; 11 (3) Aging Activates the Immune System and Alters the Regenerative Capacity in the Zebrafish Heart.
Martins RR et al. Front Cell Dev Biol 2019; 7 : 12 Resident Immunity in Tissue Repair and Maintenance: The Zebrafish Model Coming of Age.
Tyrkalska SD et al. Immunity 2019; (Epub) Inflammasome Regulates Hematopoiesis through Cleavage of the Master Erythroid Transcription Factor GATA1.
Application : WB
Reactivity : Zebrafish
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169