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HLA-DQB1 antibody

Anti-HLA-DQB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113458
Anti-HLA-DQB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113458
Anti-HLA-DQB1 antibody used in Western Blot (WB). GTX113458

Cat. No. GTX113458

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HLA-DQB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human HLA DQB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class II, DQ beta 1 , CELIAC1 , HLA-DQB , IDDM1

Background

HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-HLA-DQB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113458

GTX113458 ICC/IF Image

HLA DQB1 antibody detects HLA DQB1 protein at cell membrane and endoplasmic reticulum by immunofluorescent analysis.
Sample: Raji cells were fixed in iced-cold MeOH for 5 min.
Green: HLA DQB1 protein stained by HLA DQB1 antibody (GTX113458) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HLA-DQB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113458

GTX113458 IHC-P Image

HLA DQB1 antibody detects HLA DQB1 protein at cytoplasm and membrane on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
HLA DQB1 antibody (GTX113458) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HLA-DQB1 antibody used in Western Blot (WB). GTX113458

GTX113458 WB Image

HLA DQB1 antibody detects HLA DQB1 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
12% SDS-PAGE
HLA DQB1 antibody (GTX113458) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zhang L et al. Cancer Manag Res 2019; 11 : 2605-2616 HLA-DQB1 expression on tumor cells is a novel favorable prognostic factor for relapse in early-stage lung adenocarcinoma.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production