APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Recommended starting dilution is 1:1000. |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT3D2 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BIP protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HELS89n , HSPA5 , MIF2 , Bip , heat shock 70kDa protein 5
Cellular Localization
Endoplasmic reticulum lumen,Melanosome,Cytoplasm
Background
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
Database
Research Area
DATA IMAGES
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GTX57602 WB Image
WB analysis of various samples using GTX57602 Grp78 antibody. Samples : HeLa and MCF-7 whole cell lysate Loading : 40 μg Dilution : 1:1000
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GTX57602 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57602 Grp78 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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GTX57602 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57602 Grp78 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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REFERENCE
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REVIEW
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