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Tuba1 antibody

Anti-Tuba1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124965
Anti-Tuba1 antibody used in Western Blot (WB). GTX124965
Anti-Tuba1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124965
Anti-Tuba1 antibody used in Western Blot (WB). GTX124965

Cat. No. GTX124965

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Tuba1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

Zebrafish brain , Zebrafish eye

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Sheep (100%)| Bovine (100%)| Chimpanzee (99%)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Tuba1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172299

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin, alpha 1b , cb944 , fb22g06 , tuba1 , wu:fb22g06

Database

Research Area

DATA IMAGES

Anti-Tuba1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124965

GTX124965 IHC-Wm Image

Tuba1 antibody detects Tuba1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Tuba1 stained by Tuba1 antibody (GTX124965) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Tuba1 antibody used in Western Blot (WB). GTX124965

GTX124965 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tuba1 antibody (GTX124965) diluted at 1:1000.

Anti-Tuba1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124965

GTX124965 IHC-Wm Image

Tuba1 antibody detects Tuba1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Tuba1 stained by Tuba1 antibody (GTX124965) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Tuba1 antibody used in Western Blot (WB). GTX124965

GTX124965 WB Image

The data was published in the journal Front Mol Neurosci in 2019. PMID: 31040768

REFERENCE

Application Reference

Mans RA et al. Front Mol Neurosci 2019; 12 : 91 Cholinergic Stimulation of the Adult Zebrafish Brain Induces Phosphorylation of Glycogen Synthase Kinase-3 帣 and Extracellular Signal-Regulated Kinase in the Telencephalon.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169