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RhoB antibody

Anti-RhoB antibody used in Western Blot (WB). GTX108600
Anti-RhoB antibody used in Western Blot (WB). GTX108600
Anti-RhoB antibody used in IHC (Paraffin sections) (IHC-P). GTX108600
Anti-RhoB antibody used in Western Blot (WB). GTX108600

Cat. No. GTX108600

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RHOB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Mouse brain , U87-MG , rat brain

Predict Reactivity

Sheep, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RhoB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037877

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ras homolog family member B , ARH6 , ARHB , MST081 , MSTP081 , RHOH6

Cellular Localization

Late endosome membrane; Lipid-anchor , Cell membrane; Lipid-anchor , Nucleus

Database

Research Area

DATA IMAGES

Anti-RhoB antibody used in Western Blot (WB). GTX108600

GTX108600 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX108600 diluted at 1:1000

Anti-RhoB antibody used in Western Blot (WB). GTX108600

GTX108600 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX108600 diluted at 1:500

Anti-RhoB antibody used in IHC (Paraffin sections) (IHC-P). GTX108600

GTX108600 IHC-P Image

RhoB antibody detects RHOB protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
RhoB antibody (GTX108600) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RhoB antibody used in Western Blot (WB). GTX108600

GTX108600 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RhoB antibody (GTX108600) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169