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Elastase antibody

Anti-Elastase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21876
Anti-Elastase antibody used in Western Blot (WB). GTX21876

Cat. No. GTX21876

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Multiplexing

Reactivity

 Mouse
Package
100 μg ($589)
See all Elastase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
ELISA 1:5000-1:25000
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Elastase collected from porcine pancreas

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chymotrypsin like elastase family member 1 , ELA1 , ELS1 , Elastase-1

Cellular Localization

Secreted

Background

Acts upon elastin.

Database

Research Area

DATA IMAGES

Anti-Elastase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21876

GTX21876 ICC/IF Image

Double immunofluorescence microscopy after staining of mouse embryonic gut tissue with anti-Elastase antibody and anti-Insulin antibody. Tissue sections were prepared using frozen sections after fixation for 1 h in 4% PFA. The image shows elastase staining in green and insulin staining in red. Personal communication, C. Murtaugh, Harvard University

Anti-Elastase antibody used in Western Blot (WB). GTX21876

GTX21876 WB Image

WB analysis of recombinant mouse elastase protein using GTX21876 Elastase antibody.
Loading : 50 ng
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 589