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Caveolin 1 antibody

Anti-Caveolin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22910
Anti-Caveolin 1 antibody used in Western Blot (WB). GTX22910
Anti-Caveolin 1 antibody used in Immunoprecipitation (IP). GTX22910
Anti-Caveolin 1 antibody used in Western Blot (WB). GTX22910

Cat. No. GTX22910

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP, IHC

Reactivity

 Human, Mouse, Rat, Dog, Hamster
Package
100 μg ($399)
See all Caveolin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
ICC/IF 1-2 μg/ml
FACS 3-5 μg/10⁶ cells
IP 5 μg
IHC Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues M(1) S G G K Y V D S E G H L Y T V P(17) C of human CAV1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caveolin 1

Cellular Localization

Golgi apparatus membrane,Cell membrane

Background

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

Database

Research Area

DATA IMAGES

Anti-Caveolin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22910

GTX22910 ICC/IF Image

ICC/IF analysis of A375 cells using GTX22910 Caveolin 1 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA and incubated for 3 hours at room temperature

Anti-Caveolin 1 antibody used in Western Blot (WB). GTX22910

GTX22910 WB Image

WB analysis of whole cell extracts (20 μg lysate) of PANC-1 (Lane 1), U-87 MG (Lane 2), A549 (Lane 3), HeLa (lane4), PC-3 (lane 5), U2OS (lane 6), Mouse Heart (lane 7), Rat Heart (lane 8), C2C12 (lane 9), Mouse Lung (lane 10) and A-431 (lane 11) using GTX22910 Caveolin 1 antibody.
Dilution : 1-2 μg/ml

Anti-Caveolin 1 antibody used in Immunoprecipitation (IP). GTX22910

GTX22910 IP Image

IP analysis of mouse heart lysate using GTX22910 Caveolin 1 antibody.
Lane 1 : 10 % input
Lane 2 : Isotype control
Lane 3 : 5μg primary antibody

Anti-Caveolin 1 antibody used in Western Blot (WB). GTX22910

GTX22910 WB Image

WB analysis of rat heart protein extract using GTX22910 Caveolin 1 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399