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Nucleolin antibody [4i51]

Anti-Nucleolin antibody [4i51] used in Western Blot (WB). GTX14966

Cat. No. GTX14966

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4i51

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human
Package
25 μl ($399)
See all Nucleolin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:10-1:100
IP Assay dependent
ELISA Assay dependent

Note :

ICC/IF
Suggest to fix cells with 4% paraformaldehyde and permeabilized with 0.25% Triton-X 100.
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Nuclear matrix extract from MDCK cells.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C23 , NCL , nucleolin , Nucleolin

Cellular Localization

Nucleus,Nucleolus,Cytoplasm

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Nucleolin antibody [4i51] used in Western Blot (WB). GTX14966

GTX14966 WB Image

WB analysis of HeLa nuclear extract using GTX14966 Nucleolin antibody [4i51].
Dilution : 1:5000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399