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ARS2 antibody

Anti-ARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX119872
Anti-ARS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119872
Anti-ARS2 antibody used in Western Blot (WB). GTX119872

Cat. No. GTX119872

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ARS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ARS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720168

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serrate, RNA effector molecule , ARS2 , ASR2 , serrate

Background

Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and RNASEN, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity.

Database

Research Area

DATA IMAGES

Anti-ARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX119872

GTX119872 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ARS2(GTX119872) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119872

GTX119872 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARS2(GTX119872) antibody at 1:500 dilution.

Anti-ARS2 antibody used in Western Blot (WB). GTX119872

GTX119872 WB Image

ARS2 antibody detects ARS2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
ARS2 antibody (GTX119872) dilution: 1:1000

REFERENCE

Application Reference

Pol?k P et al. Cell Rep 2023; 42 (11) : 113325 Dual agonistic and antagonistic roles of ZC3H18 provide for co-activation of distinct nuclear RNA decay pathways.
Yin J et al. Nat Commun 2020; 11 (1) : 2978 ARS2/MAGL signaling in glioblastoma stem cells promotes self-renewal and M2-like polarization of tumor-associated macrophages.
Application : WB, ICC/IF, IP
Reactivity : Human
Lloret-Llinares M et al. Methods Mol Biol 2020; 2062 : 127-145 Global Identification of Human Exosome Substrates Using RNA Interference and RNA Sequencing.
Application : WB
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169