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PKNOX1 antibody

Orthogonal
Anti-PKNOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114991
Anti-PKNOX1 antibody used in Western Blot (WB). GTX114991

Cat. No. GTX114991

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ChIP-seq

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PKNOX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
ChIP-seq Assay dependent

Note :

IP
target validated by Mass Spectrometry
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKNOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171103

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PBX/knotted 1 homeobox 1 , PREP1 , pkonx1c

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-PKNOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114991

GTX114991 ICC/IF Image

PKNOX1 antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKNOX1 protein stained by PKNOX1 antibody (GTX114991) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-PKNOX1 antibody used in Western Blot (WB). GTX114991

GTX114991 WB Image

Orthogonal

HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKNOX1 antibody (GTX114991) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169