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RPL15 antibody

Anti-RPL15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101830
Anti-RPL15 antibody used in Western Blot (WB). GTX101830
Anti-RPL15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101830
Anti-RPL15 antibody used in Western Blot (WB). GTX101830

Cat. No. GTX101830

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RPL15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615101

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L15 , DBA12 , EC45 , L15 , RPL10 , RPLY10 , RPYL10

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10, its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101830

GTX101830 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal colon, using RPL15(GTX101830) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL15 antibody used in Western Blot (WB). GTX101830

GTX101830 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
RPL15 antibody
GTX101830 diluted at 1:1000

Anti-RPL15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101830

GTX101830 IHC-P Image

RPL15 antibody detects RPL15 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: RPL15 antibody (GTX101830) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL15 antibody used in Western Blot (WB). GTX101830

GTX101830 WB Image

The data was published in the journal Cell Death Dis in 2016. PMID: 27253413

REFERENCE

Application Reference

Sbarrato T et al. Cell Death Dis 2016; 7 (6) : e2249 A ribosome-related signature in peripheral blood CLL B cells is linked to reduced survival following treatment.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169