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BRG1 antibody [BRG-01]

Anti-BRG1 antibody [BRG-01] used in Western Blot (WB). GTX80217

Cat. No. GTX80217

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BRG-01

Isotype

 IgG2a

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all BRG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

185 kDa. ( Note )

Product Note

This antibody recognizes an epitope within C-terminal part of human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BAF190 , BAF190A , BRG1 , CSS4 , MRD16 , RTPS2 , SMARCA4 , SNF2 , SNF2L4 , SNF2LB , SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , SWI2 , hSNF2b

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-BRG1 antibody [BRG-01] used in Western Blot (WB). GTX80217

GTX80217 WB Image

WB analysis of various samples using GTX80217 BRG1 antibody [BRG-01].
Panel A : Brg1-negative SW13 cells transfected with Brg1
Panel B : HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates

REFERENCE

Application Reference

Kiminori Kurashima et al. NAR Cancer 2020; 2 (2) : zcaa005 SMARCA4 deficiency-associated heterochromatin induces intrinsic DNA replication stress and susceptibility to ATR inhibition in lung adenocarcinoma.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399