GeneTex

  • Country / Location Selection

United States (US)

MFN1 antibody

Anti-MFN1 antibody used in Western Blot (WB). GTX133351
Anti-MFN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133351
Anti-MFN1 antibody used in Western Blot (WB). GTX133351
Anti-MFN1 antibody used in Western Blot (WB). GTX133351
Anti-MFN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133351

Cat. No. GTX133351

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MFN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

mouse heart , rat kidney , human kidney

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse MFN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886948

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitofusin 1 , 2310002F04Rik , 6330416C07Rik , D3Ertd265e , HR2 , mKIAA4032

Cellular Localization

Mitochondrion outer membrane; Multi-pass membrane protein

Background

Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.

Database

Research Area

DATA IMAGES

Anti-MFN1 antibody used in Western Blot (WB). GTX133351

GTX133351 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MFN1 antibody (GTX133351) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MFN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133351

GTX133351 IHC-P Image

MFN1 antibody detects MFN1 protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
MFN1 antibody (GTX133351) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MFN1 antibody used in Western Blot (WB). GTX133351

GTX133351 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MFN1 antibody (GTX133351) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MFN1 antibody used in Western Blot (WB). GTX133351

GTX133351 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MFN1 antibody (GTX133351) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MFN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133351

GTX133351 IHC-P Image

MFN1 antibody detects MFN1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
MFN1 antibody (GTX133351) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chia-Jung Li et al. 2022; 21 : e13546 Phosphoglycerate mutase family member 5 maintains oocyte quality via mitochondrial dynamic rearrangement during aging
Chen KC et al. J Clin Med 2021; 11 (1) Bicalutamide Exhibits Potential to Damage Kidney via Destroying Complex I and Affecting Mitochondrial Dynamics.
Application : WB
Reactivity : Rat
Sakiyama H et al. Mol Cell Biochem 2021; 476 (10) : 3577-3590 A lack of ChREBP inhibits mitochondrial cristae formation in brown adipose tissue.
Yan H et al. Neuropharmacology 2021; 194 : 108627 Parecoxib alleviates the motor behavioral decline of aged rats by ameliorating mitochondrial dysfunction in the substantia nigra via COX-2/PGE2 pathway inhibition.
Application : WB
Reactivity : Rat
Ding M et al. Acta Physiol (Oxf) 2019; : e13428 Mitochondrial fusion promoter restores mitochondrial dynamics balance and ameliorates diabetic cardiomyopathy in an optic atrophy 1-dependent way.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

There are currently no reviews for MFN1 antibody (GTX133351). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169