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Tub antibody

Anti-Tub antibody used in IHC (Paraffin sections) (IHC-P). GTX118348
Anti-Tub antibody used in IHC (Paraffin sections) (IHC-P). GTX118348
Anti-Tub antibody used in Western Blot (WB). GTX118348
Anti-Tub antibody used in IHC (Paraffin sections) (IHC-P). GTX118348
Anti-Tub antibody used in Western Blot (WB). GTX118348
Anti-Tub antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118348

Cat. No. GTX118348

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TUB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH , Neuro2A , GL261

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tub. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166239

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubby bipartite transcription factor , RDOB , rd5

Background

This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Tub antibody used in IHC (Paraffin sections) (IHC-P). GTX118348

GTX118348 IHC-P Image

Tub antibody detects Tub protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Tub antibody (GTX118348) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tub antibody used in IHC (Paraffin sections) (IHC-P). GTX118348

GTX118348 IHC-P Image

Tub antibody detects Tub protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Tub antibody (GTX118348) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tub antibody used in Western Blot (WB). GTX118348

GTX118348 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
7.5% SDS PAGE
GTX118348 diluted at 1:1000

Anti-Tub antibody used in IHC (Paraffin sections) (IHC-P). GTX118348

GTX118348 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Tub(GTX118348) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tub antibody used in Western Blot (WB). GTX118348

GTX118348 WB Image

Sample (30 ug of whole cell lysate)
A: Neuro2A
B: GL261
7.5% SDS PAGE
GTX118348 diluted at 1:1000

Anti-Tub antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118348

GTX118348 ICC/IF Image

Tub antibody detects Tub protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tub protein stained by Tub antibody (GTX118348) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169