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ZNF45 antibody [N2C3]

Anti-ZNF45 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103451
Anti-ZNF45 antibody [N2C3] used in Western Blot (WB). GTX103451
Anti-ZNF45 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103451
Anti-ZNF45 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX103451

Cat. No. GTX103451

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all zinc finger protein 45 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF45. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952686

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 45 , KOX5 , ZNF13

Cellular Localization

Nucleus

Background

May be involved in transcriptional regulation.

Database

Research Area

DATA IMAGES

Anti-ZNF45 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103451

GTX103451 ICC/IF Image

ZNF45 antibody [N2C3] detects ZNF45 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF45 protein stained by ZNF45 antibody [N2C3] (GTX103451) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ZNF45 antibody [N2C3] used in Western Blot (WB). GTX103451

GTX103451 WB Image

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX103451 diluted at 1:2000

Anti-ZNF45 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103451

GTX103451 ICC/IF Image

ZNF45 antibody [N2C3] detects ZNF45 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF45 protein stained by ZNF45 antibody [N2C3] (GTX103451) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ZNF45 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX103451

GTX103451 IHC-P Image

ZNF45 antibody [N2C3] detects ZNF45 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ZNF45 antibody [N2C3] (GTX103451) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169