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SLC25A23 antibody

Anti-SLC25A23 antibody used in Western Blot (WB). GTX116402
Anti-SLC25A23 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116402
Anti-SLC25A23 antibody used in Western Blot (WB). GTX116402
Anti-SLC25A23 antibody used in IHC (Paraffin sections) (IHC-P). GTX116402

Cat. No. GTX116402

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SLC25A23 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Mouse brain , IMR32

Predict Reactivity

Rat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLC25A23. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618889

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 25 member 23 , APC2 , MCSC2 , SCaMC-3

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria.

Database

Research Area

DATA IMAGES

Anti-SLC25A23 antibody used in Western Blot (WB). GTX116402

GTX116402 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX116402 diluted at 1:1000

Anti-SLC25A23 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116402

GTX116402 ICC/IF Image

SLC25A23 antibody detects SLC25A23 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: SLC25A23 protein stained by SLC25A23 antibody (GTX116402) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SLC25A23 antibody used in Western Blot (WB). GTX116402

GTX116402 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX116402 diluted at 1:500

Anti-SLC25A23 antibody used in IHC (Paraffin sections) (IHC-P). GTX116402

GTX116402 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SLC25A23(GTX116402) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Zhang K et al. Mol Cancer 2018; 17 (1) : 153 Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Application : WB
Reactivity : Human
Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169