SAP130 antibody [C2C3], C-term

Cat. No. GTX112169

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all SAP130 products

Datasheet
Document

View product citations for antibody GTX112169 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SAP130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620318

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sin3A associated protein 130

Cellular Localization

Nucleus

Background

SAP130 is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex (Fleischer et al., 2003 [PubMed 12724404]).[supplied by OMIM]

Database

Gene ID: 79595 SAP130
UniProt: Q9H0E3 SAP130

Research Area

DATA IMAGES

GTX112169 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using SAP130(GTX112169) antibody at 1:500 dilution.

GTX112169 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX112169 diluted at 1:1000

GTX112169 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using SAP130(GTX112169) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty