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THOC7 antibody

Anti-THOC7 antibody used in Western Blot (WB). GTX119877
Anti-THOC7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119877
Anti-THOC7 antibody used in Western Blot (WB). GTX119877

Cat. No. GTX119877

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all THOC7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431 , Mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human THOC7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721273

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

THO complex 7 , NIF3L1BP1 , fSAP24 , hTREX30

Cellular Localization

Cytoplasm , Nucleus

Background

Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.

Database

Research Area

DATA IMAGES

Anti-THOC7 antibody used in Western Blot (WB). GTX119877

GTX119877 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with THOC7 antibody (GTX119877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-THOC7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119877

GTX119877 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using THOC7(GTX119877) antibody at 1:200 dilution.

Anti-THOC7 antibody used in Western Blot (WB). GTX119877

GTX119877 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX119877 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169