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ZC3H8 antibody [N1C3]

Anti-ZC3H8 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107008
Anti-ZC3H8 antibody [N1C3] used in Western Blot (WB). GTX107008
Anti-ZC3H8 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107008

Cat. No. GTX107008

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZC3H8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

A431 , HepG2 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZC3H8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952645

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger CCCH-type containing 8 , Fliz1 , ZC3HDC8

Cellular Localization

Nucleus

Background

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Database

Research Area

DATA IMAGES

Anti-ZC3H8 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107008

GTX107008 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using ZC3H8(GTX107008) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ZC3H8 antibody [N1C3] used in Western Blot (WB). GTX107008

GTX107008 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
C:Raji (GTX27908)
12% SDS PAGE
GTX107008 diluted at 1:1000

Anti-ZC3H8 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107008

GTX107008 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZC3H8(GTX107008) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169