GeneTex

  • Country / Location Selection

United States (US)

Cyclin B2 antibody

Anti-Cyclin B2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111118
Anti-Cyclin B2 antibody used in Western Blot (WB). GTX111118
Anti-Cyclin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111118
Anti-Cyclin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111118

Cat. No. GTX111118

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cyclin B2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949888

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin B2 , HsT17299

Background

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin B2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111118

GTX111118 ICC/IF Image

Cyclin B2 antibody [N1C3-2] detects CCNB2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CCNB2 protein stained by Cyclin B2 antibody [N1C3-2] (GTX111118) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-Cyclin B2 antibody used in Western Blot (WB). GTX111118

GTX111118 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX111118 diluted at 1:1000

Anti-Cyclin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111118

GTX111118 IHC-P Image

Cyclin B2 antibody detects Cyclin B2 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Cyclin B2 antibody (GTX111118) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111118

GTX111118 IHC-P Image

Cyclin B2 antibody detects CCNB2 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Cyclin B2 antibody (GTX111118) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for Cyclin B2 antibody (GTX111118). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Cyclin B2 antibody (GTX111118). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169