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RPA14 antibody [11.1]

Anti-RPA14 antibody [11.1] used in Western Blot (WB). GTX70238
Anti-RPA14 antibody [11.1] used in IHC (Paraffin sections) (IHC-P). GTX70238
Anti-RPA14 antibody [11.1] used in Western Blot (WB). GTX70238

Cat. No. GTX70238

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 11.1

Isotype

 IgG1

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all RPA14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 - 2 μg/ml
IHC-P 1:100-1:1000
IP 0.1 - 2 μg/ml
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

MCF7, T24

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human RPA-14 expressed in E. coli..

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_372932

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication protein A3 , REPA3 , RP-A p14

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-RPA14 antibody [11.1] used in Western Blot (WB). GTX70238

GTX70238 WB Image

RPA14 antibody [11.1] detects RPA14 protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg MCF-7 whole cell lysate/extract
15 % SDS-PAGE
RPA14 antibody [11.1] (GTX70238) dilution: 1:1000

Anti-RPA14 antibody [11.1] used in IHC (Paraffin sections) (IHC-P). GTX70238

GTX70238 IHC-P Image

RPA14 antibody [11.1] detects RPA14 protein at nucleus on Mahlarvu xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Mahlarvu xenograft.
RPA14 antibody [11.1] (GTX70238) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPA14 antibody [11.1] used in Western Blot (WB). GTX70238

GTX70238 WB Image

Sample (30 ug of whole cell lysate)
A: MCF7
15% SDS PAGE
GTX70238 diluted at 1:500

REFERENCE

Application Reference

Yoshihara T et al. EMBO J 2004; 23 (3) : 670-80 XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells.
Application : WB
Reactivity : Human
Meetei AR et al. Mol Cell Biol 2003; 23 (10) : 3417-26 A multiprotein nuclear complex connects Fanconi anemia and Bloom syndrome.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations