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GFP antibody (AP)

Anti-GFP antibody (AP) used in Western Blot (WB). GTX26661
Anti-GFP antibody (AP) used in Dot blot (Dot). GTX26661
Anti-GFP antibody (AP) used in Western Blot (WB). GTX26661
Anti-GFP antibody (AP) used in IHC (Frozen sections) (IHC-Fr). GTX26661

Cat. No. GTX26661

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, Dot, ELISA, IHC

Reactivity

 Species independent
Package
100 μg ($399)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2500
ICC/IF 1:200-1:1000
IHC-Fr Assay dependent
Dot Assay dependent
ELISA 1:2000-1:8000
IHC 1:200-1:1000
Not tested in other applications.

Product Note

No reaction was observed against Human, Mouse and Rat Serum Proteins.

PROPERTIES

Form

Liquid

Buffer

50mM Tris-HCl, 150mM NaCl, 1mM MgCl₂, 0.1mM ZnCl₂, 1% BSA, 50% Glycerol

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Jellyfish

Immunogen

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Alkaline Phosphatase (AP)

RRID

AB_371411

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.

Research Area

DATA IMAGES

Anti-GFP antibody (AP) used in Western Blot (WB). GTX26661

GTX26661 WB Image

Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody (GTX26661). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none

Anti-GFP antibody (AP) used in Dot blot (Dot). GTX26661

GTX26661 Dot Image

Dot blot analysis of GFP using GTX26661 GFP antibody (AP).
Lane 1 : 100 ng
Lane 2 : 33.33 ng
Lane 3 : 11.11 ng
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1:1000

Anti-GFP antibody (AP) used in Western Blot (WB). GTX26661

GTX26661 WB Image

WB analysis of recombinant GFP protein using GTX26661 GFP antibody (AP).
Loading : 50 ng
Dilution : 1:1000

Anti-GFP antibody (AP) used in IHC (Frozen sections) (IHC-Fr). GTX26661

GTX26661 IHC-Fr Image

The data was published in the journal BMC Biotechnol in 2013.PMID: 24073829

REFERENCE

Application Reference

Pavoni E et al. BMC Biotechnol 2013; 13 : 79 Simultaneous display of two large proteins on the head and tail of bacteriophage lambda.
Application : ELISA
Submit a Reference

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399