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FANCD2 antibody

Anti-FANCD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX30142
Anti-FANCD2 antibody used in Western Blot (WB). GTX30142
Anti-FANCD2 antibody used in Western Blot (WB). GTX30142

Cat. No. GTX30142

Reference 12

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Dog, Primate
Package
50 μl ($449)
See all FANCD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:10000 - 1:20000
IHC-P 2.5 - 5 μg/ml
FACS Assay dependent
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

164 kDa. ( Note )

Product Note

This antibody is specific for FANCD2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_370446

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FA complementation group D2 , FA-D2 , FA4 , FACD , FAD , FAD2 , FANCD

Cellular Localization

Nucleus

Background

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FANCD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX30142

GTX30142 IHC-P Image

IHC-P analysis of human prostate tissue using GTX30142 FANCD2 antibody.

Anti-FANCD2 antibody used in Western Blot (WB). GTX30142

GTX30142 WB Image

WB analysis of HeLa cell lysate using GTX30142 FANCD2 antibody.

Anti-FANCD2 antibody used in Western Blot (WB). GTX30142

GTX30142 WB Image

WB analysis of HeLa cell lysate using GTX30142 FANCD2 antibody.
Dilution : 1:20000

REFERENCE

Application Reference

Oberholster L et al. Microbiol Spectr 2023; 11 (6) : e0275123 Comprehensive proteomic analysis of JC polyomavirus-infected human astrocytes and their extracellular vesicles.
Niraj J et al. Nucleic Acids Res 2017; 45 (14) : 8341-8357 The identification of FANCD2 DNA binding domains reveals nuclear localization sequences.
Huehls AM et al. Mol Pharmacol 2016; 89 (1) : 53-62 genomically incorporated 5-* that escapes ung-initiated base excision repair blocks dna replication and activates homologous recombination.
Application : WB
Reactivity : Human
Truong LN et al. J Biol Chem 2014; 289 (42) : 28910-23 Homologous recombination is a primary pathway to repair DNA double-strand breaks generated during DNA rereplication.
Application : WB
Reactivity : Human
Castillo A et al. Cell Rep 2014; 8 (3) : 807-17 The BRCA1-Interacting Protein Abraxas Is Required for Genomic Stability and Tumor Suppression.
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
Oberholster L et al. Microbiol Spectr 2023; 11 (6):e0275123 Comprehensive proteomic analysis of JC polyomavirus-infected human astrocytes and their extracellular vesicles.
Niraj J et al. Nucleic Acids Res 2017; 45 (14):8341-8357 The identification of FANCD2 DNA binding domains reveals nuclear localization sequences.
Huehls AM et al. Mol Pharmacol 2016; 89 (1):53-62 genomically incorporated 5-* that escapes ung-initiated base excision repair blocks dna replication and activates homologous recombination.
Application : WB
Reactivity : Human
Truong LN et al. J Biol Chem 2014; 289 (42):28910-23 Homologous recombination is a primary pathway to repair DNA double-strand breaks generated during DNA rereplication.
Application : WB
Reactivity : Human
Castillo A et al. Cell Rep 2014; 8 (3):807-17 The BRCA1-Interacting Protein Abraxas Is Required for Genomic Stability and Tumor Suppression.
Application : WB
Reactivity : Mouse
Liu R et al. Chin Med J (Engl) 2013; 126 (23):943575 Diagnosis of Fanconi anemia in children with atypical clinical features: a primary study.
Application : WB
Reactivity : Human
Geng L et al. J Cell Biol 2010; 191 (2):249-57 RAD18-mediated ubiquitination of PCNA activates the Fanconi anemia DNA repair network.
Reactivity : Human
Boichuk S et al. J Virol 2010; 84 (16):8007-20 Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
Reactivity : Human
Hicks JK et al. Mol Cell Biol 2010; 30 (5):1217-30 Differential roles for DNA polymerases eta, zeta, and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links.
Reactivity : Human
Wagner JM et al. Mol Pharmacol 2009; 76 (1):208-14 *-induced dna damage activates replication checkpoint signaling components that differentially affect tumor cell survival.
Application : WB
Reactivity : Human
Spardy N et al. Cancer Res 2008; 68 (23):9954-63 HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies.
Reactivity : Human
Spardy N et al. J Virol 2007; 81 (23):13265-70 The human papillomavirus type 16 E7 oncoprotein activates the Fanconi anemia (FA) pathway and causes accelerated chromosomal instability in FA cells.
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449