GeneTex

  • Country / Location Selection

United States (US)

nm23-H1 antibody [N1C3]

KO/KD
Anti-nm23-H1 antibody [N1C3] used in Western Blot (WB). GTX112988
Anti-nm23-H1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112988
Anti-nm23-H1 antibody [N1C3] used in Western Blot (WB). GTX112988
Anti-nm23-H1 antibody [N1C3] used in Western Blot (WB). GTX112988

Cat. No. GTX112988

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all nm23-H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

Mouse brain , HepG2 , Molt-4 , 293T

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Guinea pig, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human nm23-H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951003

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NME/NM23 nucleoside diphosphate kinase 1 , AWD , GAAD , NB , NBS , NDKA , NDPK-A , NDPKA , NM23 , NM23-H1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-nm23-H1 antibody [N1C3] used in Western Blot (WB). GTX112988

GTX112988 WB Image

KO/KD

Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody [N1C3] (GTX112988) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-nm23-H1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112988

GTX112988 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using nm23-H1(GTX112988) antibody at 1:100 dilution.

Anti-nm23-H1 antibody [N1C3] used in Western Blot (WB). GTX112988

GTX112988 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
12% SDS PAGE
GTX112988 diluted at 1:1000

Anti-nm23-H1 antibody [N1C3] used in Western Blot (WB). GTX112988

GTX112988 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112988 diluted at 1:1000

REFERENCE

Application Reference

Fa KN et al. Int J Biol Macromol 2015; 74 : 476-82 Anti-metastatic effects of antrodan, the Antrodia cinnamomea mycelia glycoprotein, in lung carcinoma cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for nm23-H1 antibody [N1C3] (GTX112988). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169