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PGP9.5 antibody

KO/KD Orthogonal
Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109637
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

Cat. No. GTX109637

Reference 13
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PGP9.5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Neuro2A , GL261 , rat brain , mouse brain

Predict Reactivity

Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PGP9.5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952497

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin C-terminal hydrolase L1 , HEL-117 , HEL-S-53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , SPG79 , Uch-L1

Cellular Localization

Cytoplasm

Background

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109637

GTX109637 ICC/IF Image

PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637

GTX109637 IHC-Fr Image

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

GTX109637 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse skin.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637

GTX109637 IHC-Fr Image

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

GTX109637 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded rat colon.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:10000.

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109637

GTX109637 ICC/IF Image

PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 stained by PGP9.5 antibody (GTX109637) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

PGP9.5 antibody detects PGP9.5 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
12% SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

PGP9.5 antibody detects PGP9.5 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:5000

Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

GTX109637 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PGP9.5 antibody (GTX109637) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

GTX109637 IHC-P Image

The data was published in the journal Cells in 2020. PMID: 32155948

REFERENCE

Application Reference

Clayton DR et al. Am J Physiol Renal Physiol 2022; 323 (3) : F299-F321 Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA(+) interstitial cells are fibroblasts.
Chang WY et al. Lab Invest 2022; 102 (9) : 1023-1037 5-HT7 receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome.
Application : IHC-Fr
Reactivity : Human, Mouse
Fede C et al. Sci Rep 2021; 11 (1) : 12623 Evidence of a new hidden neural network into deep fasciae.
Application : IHC-P
Reactivity : Mouse
Koshimizu H et al. Life Sci 2020; 263 : 118577 Zonisamide ameliorates neuropathic pain partly by suppressing microglial activation in the spinal cord in a mouse model.
Application : ICC/IF
Reactivity : Rat
Hong J et al. Neurosci Lett 2020; 737 : 135320 Identification of Cardiac Expression Pattern of Transient Receptor Potential Vanilloid Type 1 (TRPV1) Receptor using a Transgenic Reporter Mouse Model.
Application : IHC-Fr
Reactivity : Mouse
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Submit a Reference

REVIEW

PGP9.5 antibody Cat. No. GTX109637

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637
Date : Anonymous submitted on 01-Aug-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Dorsal root ganglion
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.3% Triton X-100 in PBS, 25°C
Primary Antibody : 1:500, 4°C, 6Hr
Figure Note : PGP9.5 (left panel), and counterstain with hoechst (right panel).
Submit a Review
Application Reference
Clayton DR et al. Am J Physiol Renal Physiol 2022; 323 (3):F299-F321 Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA(+) interstitial cells are fibroblasts.
Chang WY et al. Lab Invest 2022; 102 (9):1023-1037 5-HT7 receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome.
Application : IHC-Fr
Reactivity : Human, Mouse
Fede C et al. Sci Rep 2021; 11 (1):12623 Evidence of a new hidden neural network into deep fasciae.
Application : IHC-P
Reactivity : Mouse
Koshimizu H et al. Life Sci 2020; 263:118577 Zonisamide ameliorates neuropathic pain partly by suppressing microglial activation in the spinal cord in a mouse model.
Application : ICC/IF
Reactivity : Rat
Hong J et al. Neurosci Lett 2020; 737:135320 Identification of Cardiac Expression Pattern of Transient Receptor Potential Vanilloid Type 1 (TRPV1) Receptor using a Transgenic Reporter Mouse Model.
Application : IHC-Fr
Reactivity : Mouse
Canta A et al. Antioxidants (Basel) 2020; 9 (7)
Application : IHC-P
Reactivity : Mouse
Dragunas G et al. FASEB J 2020; 34 (6):7703-7717 Cholinergic neuroplasticity in asthma driven by TrkB signaling.
Application : IHC-Fr
Reactivity : Mouse
Pagella P et al. Cells 2020; 9 (3) Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons.
Application : IHC-P
Reactivity : Human
Alberti P et al. Neuropharmacology 2020; 164:107905
Application : IHC-P
Reactivity : Rat
Xu YM et al. Toxicol Res (Camb) 2019; 8 (6):994-1001 Cadmium telluride quantum dot-exposed human bronchial epithelial cells: a further study of the cellular response by proteomics.
Meregalli C et al. J Neuroinflammation 2018; 15 (1):232 high-dose intravenous immunoglobulins reduce nerve macrophage infiltration and the severity of *-induced peripheral neurotoxicity in rats.
Application : IHC-P
Reactivity : Rat
Tu NH et al. Eur J Neurosci 2017; Na+ /K+ -ATPase coupled to endothelin receptor type B stimulates peripheral nerve regeneration via lactate signaling.
Application : ICC/IF
Reactivity : Mouse
Kishi C et al. Biol Res Nurs 2015; 17 (2):135-41 Hypo-osmotic shock-induced subclinical inflammation of skin in a rat model of disrupted skin barrier function.
Application : IHC-Fr
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169