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FUBP1 antibody

Orthogonal
Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115154
Anti-FUBP1 antibody used in Western Blot (WB). GTX115154
Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115154
Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115154
Anti-FUBP1 antibody used in Western Blot (WB). GTX115154
Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115154
Anti-FUBP1 antibody used in Western Blot (WB). GTX115154

Cat. No. GTX115154

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FUBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FUBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729580

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

far upstream element binding protein 1 , FBP , FUBP , hDH V

Cellular Localization

Nucleus

Background

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115154

GTX115154 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using FUBP1(GTX115154) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FUBP1 antibody used in Western Blot (WB). GTX115154

GTX115154 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX115154) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115154

GTX115154 ICC/IF Image

FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FUBP1 protein stained by FUBP1 antibody (GTX115154) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115154

GTX115154 ICC/IF Image

FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FUBP1 protein stained by FUBP1 antibody (GTX115154) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-FUBP1 antibody used in Western Blot (WB). GTX115154

GTX115154 WB Image

Orthogonal

Hela whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX115154) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115154

GTX115154 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using FUBP1(GTX115154) antibody at 1:500 dilution.

Anti-FUBP1 antibody used in Western Blot (WB). GTX115154

GTX115154 WB Image

Orthogonal

The data was published in the journal PLoS Pathog in 2016. PMID: 27780225

REFERENCE

Application Reference

Hung CT et al. PLoS Pathog 2016; 12 (10) : e1005959 Additive Promotion of Viral Internal Ribosome Entry Site-Mediated Translation by Far Upstream Element-Binding Protein 1 and an Enterovirus 71-Induced Cleavage Product.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : ICC/IF, IP
Reactivity : Human
Chang JF et al. Toxicol Sci 2014; (Epub) nuclear proteomics with xrcc3 knockdown to reveal the development of *-resistant uterine cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169