ELMO1 antibody [C3], C-term

Name: ELMO1 antibody [C3], C-term
Brand: GeneTex
SKU: GTX106301
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-ELMO1 antibody [C3], C-term used in Western Blot (WB). GTX106301

Anti-ELMO1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106301

Anti-ELMO1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106301

Cat. No. GTX106301

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all ELMO1 products

Datasheet
Document

View product citations for antibody GTX106301 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

H1299 , PC-12

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ELMO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240760

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

engulfment and cell motility 1 , CED-12 , CED12 , ELMO-1

Cellular Localization

Cytoplasm , Cell membrane

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Gene ID: 9844 ELMO1
UniProt: Q92556 ELMO1

Research Area

DATA IMAGES

GTX106301 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX106301 diluted at 1:1000

GTX106301 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human stomach, using ELMO1(GTX106301) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106301 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ELMO1(GTX106301) antibody at 1:500 dilution.

GTX106301 WB Image

ELMO1 antibody [C3], C-term detects ELMO1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
7.5% SDS-PAGE
ELMO1 antibody [C3], C-term (GTX106301) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106301 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
12% SDS PAGE
GTX106301 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106301 WB Image

ELMO1 antibody [C3], C-term detects ELMO1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
ELMO1 antibody [C3], C-term (GTX106301) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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