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ERH antibody

Anti-ERH antibody used in IHC (Whole mount) (IHC-Wm). GTX101806
Anti-ERH antibody used in IHC (Paraffin sections) (IHC-P). GTX101806
Anti-ERH antibody used in Western Blot (WB). GTX101806
Anti-ERH antibody used in Western Blot (WB). GTX101806
Anti-ERH antibody used in IHC (Paraffin sections) (IHC-P). GTX101806

Cat. No. GTX101806

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ERH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Mouse brain , A549 , H1299 , HCT116

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950225

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERH mRNA splicing and mitosis factor , DROER

Background

May have a role in the cell cycle.

Database

Research Area

DATA IMAGES

Anti-ERH antibody used in IHC (Whole mount) (IHC-Wm). GTX101806

GTX101806 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using ERH antibody (GTX101806) at 1:200 dilution.

Anti-ERH antibody used in IHC (Paraffin sections) (IHC-P). GTX101806

GTX101806 IHC-P Image

ERH antibody detects ERH protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ERH antibody (GTX101806) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERH antibody used in Western Blot (WB). GTX101806

GTX101806 WB Image

ERH antibody detects ERH protein by western blot analysis.
A. 30 μg A549 whole cell extract
B. 30 μg H1299 whole cell extract
C. 30 μg HCT116 whole cell extract
15 % SDS-PAGE
ERH antibody (GTX101806) dilution: 1:500

Anti-ERH antibody used in Western Blot (WB). GTX101806

GTX101806 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX101806 diluted at 1:1000

Anti-ERH antibody used in IHC (Paraffin sections) (IHC-P). GTX101806

GTX101806 IHC-P Image

ERH antibody detects ERH protein at cytosol on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
ERH antibody (GTX101806) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169