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GCSH antibody

Anti-GCSH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66196
Anti-GCSH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66196
Anti-GCSH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66196
Anti-GCSH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66196
Anti-GCSH antibody used in Western Blot (WB). GTX66196

Cat. No. GTX66196

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GCSH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-173 of human GCSH (NP_004474.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycine cleavage system protein H , GCE , NKH

Cellular Localization

Mitochondrion

Background

Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for this gene. Also, several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.[provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-GCSH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66196

GTX66196 ICC/IF Image

ICC/IF analysis of C6 cells using GTX66196 GCSH antibody.
Blue : DAPI
Dilution : 1:100

Anti-GCSH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66196

GTX66196 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66196 GCSH antibody.
Blue : DAPI
Dilution : 1:100

Anti-GCSH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66196

GTX66196 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66196 GCSH antibody.
Blue : DAPI
Dilution : 1:100

Anti-GCSH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66196

GTX66196 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66196 GCSH antibody.
Blue : DAPI
Dilution : 1:100

Anti-GCSH antibody used in Western Blot (WB). GTX66196

GTX66196 WB Image

WB analysis of various sample lysates using GTX66196 GCSH antibody.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399